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overexpression plasmid pet28a  (Addgene inc)


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    Structured Review

    Addgene inc overexpression plasmid pet28a
    Overexpression Plasmid Pet28a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/overexpression+plasmid+pet28a/pET28a-sfGFP+(Plasmid+%2385492)/pm39177329-148-1-4
    Average 93 stars, based on 15 article reviews
    overexpression plasmid pet28a - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: Dual feedback inhibition of ATP-dependent caffeate activation economizes ATP in caffeate-dependent electron bifurcation.
    Article Snippet: The acetogen Acetobacterium woodii couples caffeate reduction with ferredoxin reduction and NADH oxidation via electron bifurcation, providing additional reduced ferredoxin for energy conservation and cell synthesis.. Caffeate is first activated by an acyl-CoA synthetase (CarB), which ligates CoA to caffeate at the expense of ATP.. After caffeoyl-CoA is reduced to hydrocaffeoyl-CoA, the CoA moiety in hydrocaffeoyl-CoA could be recycled for caffeoyl-CoA synthesis by an ATP-independent CoA transferase (CarA) to save energy.

    Plasmid Preparation:

    Article Title: Dual feedback inhibition of ATP-dependent caffeate activation economizes ATP in caffeate-dependent electron bifurcation.
    Article Snippet: The acetogen Acetobacterium woodii couples caffeate reduction with ferredoxin reduction and NADH oxidation via electron bifurcation, providing additional reduced ferredoxin for energy conservation and cell synthesis.. Caffeate is first activated by an acyl-CoA synthetase (CarB), which ligates CoA to caffeate at the expense of ATP.. After caffeoyl-CoA is reduced to hydrocaffeoyl-CoA, the CoA moiety in hydrocaffeoyl-CoA could be recycled for caffeoyl-CoA synthesis by an ATP-independent CoA transferase (CarA) to save energy.

    Purification:

    Article Title: Dual feedback inhibition of ATP-dependent caffeate activation economizes ATP in caffeate-dependent electron bifurcation.
    Article Snippet: The acetogen Acetobacterium woodii couples caffeate reduction with ferredoxin reduction and NADH oxidation via electron bifurcation, providing additional reduced ferredoxin for energy conservation and cell synthesis.. Caffeate is first activated by an acyl-CoA synthetase (CarB), which ligates CoA to caffeate at the expense of ATP.. After caffeoyl-CoA is reduced to hydrocaffeoyl-CoA, the CoA moiety in hydrocaffeoyl-CoA could be recycled for caffeoyl-CoA synthesis by an ATP-independent CoA transferase (CarA) to save energy.

    Gel Extraction:

    Article Title: Dual feedback inhibition of ATP-dependent caffeate activation economizes ATP in caffeate-dependent electron bifurcation.
    Article Snippet: The acetogen Acetobacterium woodii couples caffeate reduction with ferredoxin reduction and NADH oxidation via electron bifurcation, providing additional reduced ferredoxin for energy conservation and cell synthesis.. Caffeate is first activated by an acyl-CoA synthetase (CarB), which ligates CoA to caffeate at the expense of ATP.. After caffeoyl-CoA is reduced to hydrocaffeoyl-CoA, the CoA moiety in hydrocaffeoyl-CoA could be recycled for caffeoyl-CoA synthesis by an ATP-independent CoA transferase (CarA) to save energy.



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    Image Search Results


    Plasmids Used in This Study

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: Heterologous Naringenin Production in the Filamentous Fungus Penicillium rubens

    doi: 10.1021/acs.jafc.3c06755

    Figure Lengend Snippet: Plasmids Used in This Study

    Article Snippet: pET28a/Cas9-Cy , Cas9 protein overexpression , , Addgene#53261 .

    Techniques: Cloning, Plasmid Preparation, Selection, Marker, Over Expression